Brian W. Kirkpatrick

Professor - Molecular Genetics

60% Research, 40% Teaching

Phone

608-263-4323

Office Location

1675 Observatory Dr
542 Animal Sciences
Madison, WI 53706

Brian Kirkpatrick is a Professor in the Department of Animal and Dairy Sciences whose research and instructional activities related to genetics and genomics of livestock.  Dr. Kirkpatrick’s research activities focus on understanding the genetic basis for variation in health and reproduction in both beef and dairy cattle.  Within these areas, the genetics of host susceptibility to infection by Mycobacterium avium subspecies paratuberculosis, the cause of Johne’s disease, and genetics of ovulation rate and twinning rate are specific emphases.  

Selected Peer-Reviewed Articles

Kirkpatrick BW, Cooke ME, Frie M, Sporer KRB, Lett B, Wells SJ, Coussens PM. 2022. Genome-wide association analysis for susceptibility to infection by Mycobacterium avium ssp. paratuberculosis in US Holsteins. J Dairy Sci. 105(5):4301-4313. doi: 10.3168/jds.2021-21276. PubMed PMID: 35307176.

Lett BM, Kirkpatrick BW. 2022. Identifying genetic variants and pathways influencing daughter averages for twinning in North American Holstein cattle and evaluating the potential for genomic selection. J Dairy Sci. 105(7):5972-5984. doi: 10.3168/jds.2021-21238. PubMed PMID: 35525609.

Madureira, Guilherme, Victor Gomez-León, Gustavo Fernandes Grillo, João Paulo Nascimento Andrade, Beth Lett, Sadrollah Molaei Moghbeli, Milo C. Wiltbank and Brian W. Kirkpatrick.  2020.  Practical application of an impractical bovine genotype; creating bilateral twin pregnancies in Trio allele carriers.  Journal of Animal Science 98(9):skaa292. doi: 10.1093/jas/skaa292.

Gomez-León, Victor E., João Paulo Andrade, Brian W. Kirkpatrick, Sadrollah Molaei Moghbeli, Alvaro Garcia-Guerra, O. J. Ginther, Milo C. Wiltbank.  2020.  Gonadotropin pulses are necessary to increase number of dominant follicles in Trio carrier heifers.  Biology of Reproduction 103(6):1217-1228. doi: 10.1093/biolre/ioaa167.

Kirkpatrick, B.W., R.M. Thallman and L.A. Kuehn.  2019.  Validation of SNP associations with bovine ovulation and twinning rate.  Animal Genetics 50:259-261

Kamalludin, Mamat H., Alvaro Garcia-Guerra, Milo C. Wiltbank, and Brian W. Kirkpatrick. 2018. Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle. Reproduction, Fertility and Development, 30:1643-1650, doi: 10.1071/RD17252. 

Frie, Meredith C.  Brian W. Kirkpatrick, Kelly R. B. Sporer, Paul M. Coussens. 2017. T and B cell activation profiles from cows with and without Johne’s disease in response to in vitro stimulation with Mycobacterium avium subspecies paratuberculosis.  Veterinary Immunology and Immunopathology 193-194:50-56.  

Kamalludin, M.H., A. Garcia-Guerra, M.C. Wiltbank, and B.W. Kirkpatrick.  2018.  Trio, a novel high fecundity allele: I. Transcriptome analysis of granulosa cells from carriers and non-carriers of the Trio allele.  Biology of Reproduction. doi: 10.1093/biolre/iox133 

Garcia-Guerra, A., M.H. Kamalludin, B.W. Kirkpatrick and M.C. Wiltbank.  2018. Trio, a bovine high fecundity allele: II. Hormonal profile and follicular dynamics underlying the high ovulation rate. Biology of Reproduction. doi: 10.1093/biolre/iox156  

Garcia-Guerra, A.M.O. Canavessi, P.L.J. Monteiro Jr., M.A. Mezera, R. Sartori, B.W. Kirkpatrick and M.C. Wiltbank. 2018.  Trio, a novel bovine high fecundity allele: III. Acquisition of dominance and ovulatory capacity at a smaller follicle size.  Biology of Reproduction. doi: 10.1093/biolre/iox157 

Baker LA, B.W. Kirkpatrick, G.J. Rosa, D. Gianola, B. Valente, J.P. Sumner, W. Baltzer, Z. Hao, E.E. Binversie, N. Volstad, A. Piazza, S. J. Sample, and P. Muir. 2017. Genome-wide association analysis in dogs implicates 99 loci as risk variants for anterior cruciate ligament rupture. PLoS One 12(4):e0173810. 

Young, A.S. and B.W. Kirkpatrick.  2016. Frequency of leukochimerism in Holstein and Jersey twinsets.  Journal of Animal Science 94:4507-15. 

Alpay, F., Y. Zare, M.H. Kamalludin, X. Huang, X. Shi, G. E. Shook, M. T. Collins, and B. W. Kirkpatrick.  2014.  Genome-wide association study of susceptibility to infection by Mycobacterium avium subspecies paratuberculosis in Holstein cattle.  PLoS ONE 9(12): e111704. doi:10.1371/journal.pone.0111704 

Zare Y., G.E. Shook, M.T. Collins and B.W Kirkpatrick. 2014. Genome-Wide Association Analysis and Genomic Prediction of Mycobacterium avium Subspecies paratuberculosis Infection in US Jersey Cattle. PLoS One. 2014 Feb 11;9(2):e88380 

Zare, Y., G.E. Shook, M.T. Collins and B.W. Kirkpatrick.  2013.  Evidence of seasonality and birth clusters of Mycobacterium avium subspecies paratuberculosis infection in US dairy herds. Preventive Veterinary Medicine 112(3-4):276-84. 

Selected Book Chapters

Kirkpatrick, B.W. 2014. Genetics of Reproduction in Cattle. Chapter 12 in The Genetics of Cattle. Ed. A. Ruvinsky and D. Garrick. CAB International, Wallinford, Oxon, UK, pp. 260-283. 

Kirkpatrick, B.W. 2014. Single Genes in Animal Breeding. Chapter 18 in Molecular and Quantitative Genetics. Ed. H. Khatib. CAB International, Wallinford, Oxon, UK, pp. 177-186 

Kirkpatrick, B.W.  2009.  Genetics of Host susceptibility to Paratuberculosis.  Chapter 6 in Paratuberculosis: Organism, Disease, Control.  Ed. D. Collins and M. Behr.  CAB International, Wallinford, Oxon, UK pp. 50-59. 

Kirkpatrick, B.W. 1999. Genetics and biology of reproduction in cattle. Chapter 13 in The Genetics of Cattle. Ed. A. Ruvinsky and R. Fries. CAB International, Wallinford, Oxon, UK pp. 391-410.

Kirkpatrick BW, Rutledge JJ.  1990.  Genetics and Transgenetics of Growth, In: Growth Regulation in Farm Animals.  Advances in Meat Research, Volume 7, A. M. Pearson, Editor. 

Selected Conference Proceedings

Kirkpatrick, B.W., M.E. Cooke, M. Frie, K.R.B. Sporer, P.M. Coussens, & S.J. Wells.  2018. Genome-wide association analysis for infection by Mycobacterium avium subspecies paratuberculosis in US Holsteins.  11th World Congress on Genetics Applied to Livestock Production. 

Kirkpatrick, B.W. and C. Morris.  2014.  Mapping of a major gene for bovine ovulation rate. 10th World Congress on Genetics Applied to Livestock Production. 

Kirkpatrick, B.W.  2002.  QTL and candidate gene effects on reproduction in livestock: Progress and Prospects. Proceedings of the 7th World Congress on Genetics Applied to Livestock Production, CD-ROM communication no 08-01. 

Kirkpatrick, B.W., X. Shi, G. Shook and M. Collins.  2010.  Whole genome association analysis of susceptibility to paratuberculosis in Holstein cattle. Proceedings of the 9th World Congress on Genetics Applied to Livestock Production, CD-ROM communication no 0939. ISBN 978-3-00-031608-1.  

Kirkpatrick, B.W.  2001.  Management of twinning cow herds.  Journal of Animal Science 80(E. Suppl. 2):E14–E18. 

Casas-Carrillo, E., A. Prill-Adams, S.G. Price and B.W. Kirkpatrick.  1994.  Association of growth hormone and insulin-like growth factor-1 genotypes with growth and carcass traits in offspring of purebred swine.  Proceedings of the 5th World Congress on Genetics Applied to Livestock Production, Vol. 21, p 272-5 

Selected Posters

Lett, B., B.W. Kirkpatrick.  2018.  Genome-wide association study of twinning rate in Holstein cattle.  Plant and Animal Genome Meeting XXVI 

Quick, A.E., T. L. Ollivett, B. W. Kirkpatrick, K. A. Weigel.  2018.  Genome wide association study and whole genome selection for resistance to bovine respiratory disease in pre-weaned Holstein calves using thoracic ultrasonography.  European Association of Animal Production. 

García-Guerra, Alvaro, Pedro L. J. Monetiro Jr., Caio A. Gamarra, Emil A. Walleser, Brian W. Kirkpatrick, Milo C. Wiltbank. 2018. Multiple dominant follicles are required for complete suppression of circulating FSH in a high fecundity bovine genotype as demonstrated with a one-follicle model.  Society for the Study of Reproduction,  

Kirkpatrick, B.W., M.H. Kamalludin, A. Garcia-Guerra, M.C. Wiltbank.  2018.  Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle.  Society for the Study of Reproduction, 458 

Young, A., B.W. Kirkpatrick.  2017.  Frequency of leukochimerism in Holstein and Jersey twinsets. Plant and Animal Genome Meeting XXV 

García-Guerra, Alvaro, Jéssica C. L. Motta, Leonardo F. Melo, Brian Kirkpatrick, Milo C. Wiltbank.  2017.  Antral Follicle Count, and Circulating Anti-Müllerian Hormone in Trio Allele Carriers, a Novel High Fecundity Bovine Genotype. Society for the Study of Reproduction, P372. 

Wiltbank, Milo C., Alvaro García-Guerra, Brian W. Kirkpatrick.  2017.  Follicular Waves and Hormonal Profiles during the Estrous Cycle of Carriers and Non-Carriers of the Trio Allele, a Major Bovine Gene for High Ovulation and Fecundity.  Society for the Study of Reproduction, P368.

Kirkpatrick, B.W., D.P. Berry.  2016.  Mapping a gene for harelip in cattle. Plant and Animal Genome Meeting XXIV 

Sallam, A.M., Y. Zare, F. Alpay, G. E. Shook, M. T. Collins, S.  Alsheikh, M. Sharaby, and B. W. Kirkpatrick. 2015. An Across-Breed Genome Wide Association Analysis of Susceptibility to Paratuberculosis in Dairy Cattle. Plant and Animal Genome Meeting.   

Kirkpatrick, BW, Morris, CA.  2012. Discovery of a major gene for bovine ovulation rate.  Plant and Animal Genome XX Conference, San Diego, CA, January 15-17, 2012, abstract P0572 

Patents 

U.S. Patent No. 5,759,772 — Method for determining the sex of an embryo, B.W. Kirkpatrick and R.L. Monson, Issued June 2, 1998.

U.S. Patent No. 10,337,067 — Compositions and Methods for Determining Likelihood of Twinning, Kirkpatrick, B.W., E.-S. Kim and C. Bierman. Kirkpatrick, Issued July 2, 2019.

U.S. Patent No. 10,745,757 — Compositions and Methods for Determining Likelihood of an Increased Susceptilbility to Contract Johne’s Disease, Kirkpatrick, B.W., G.E. Shook and M. Collins, Issued August 18, 2020.

U.S. Patent No. 11,096,382 — Methods and compositions for determining bovine ovulation rate. B.W. Kirkpatrick, Issued August 24, 2021.

Undergraduate Courses

An Sci/Dy Sci 361 – Introduction to Animal Breeding & Veterinary Genetics (2 credits, Spring)
Course Description: The molecular basis for inheritance of monogenic and polygenic traits related to animal disease and production. An introduction to the principles of improving animal health and performance by selection and mating systems in companion animals, horses, livestock, and poultry.
Requirements: Genetics 160 or 466 or con reg course in statistics

An Sci/Dy Sci 362 – Introduction to Animal Breeding & Veterinary Genetics (2 credits, Spring)
Description: The genetic basis for predisposition to diseasse or resistance to disease in livestock and companion animal species. Genetic defects, their discovery, diagnosis and treament.
Requirements: Dy Sci/AN SCI/​DY SCI  361; or Genetics 160 or 466 Zool/Bot 152

An Sci 431 – Beef Production (3 credits, Fall)
Description: Application of genetics, systems of mating, physiology, nutrition and economics to the production of beef.
Requirements: Prior completion of DY SCI/​AN SCI  313 highly recommended.

An Sci 435 – Animal Sciences Proseminar (2 credits, Fall)
Description: Methods of assessing information quality are studied. Each student develops an analytical and critical seminar on a topic of personal interest in the animal sciences.
Requirements: Senior Standing

Graduate Courses

An Sci/Dy Sci/Genetics 951 – Animal Breeding Seminar (1 credit, Spring)
Description: Review of the current scientific literature relating to animal breeding.
Requirements: Graduate/professional standing

Animal and Dairy Sciences Climate and Diversity Committee
Animal and Dairy Sciences Research and Graduate Education Committee, Chair
All Campus Animal Planning and Advisory Committee
BARD US-Israel Agricultural Research and Development Fund Technical Advisory Committee
Co-advisor of Saddle and Sirloin Club 

B.W. Kirkpatrick and R.L. Monson, "Method for determining the sex of an embryo", U.S. Patent No. 5,759,772.  The patent describes a method of sex determination which is sensitive and repeatable when applied to DNA from as little as one or two cells from a bovine embryo.  Kim, E.-S. and B.W. Kirkpatrick, 2008. Compositions And Methods For Determining Likelihood Of Twinning, P08184US (48502-418283), Provisional patent application filed 09-26-2008.  Kirkpatrick, B.W., M. Collins and G.E. Shook, 2009. Compositions and methods for determining likelihood of an increased susceptibility to contract Johne’s disease, (application number 61232428), Provisional patent application filed 08-08-2009.  Kirkpatrick, B.W., 2013. Methods and compositions for determining bovine ovulation rate, (application number 61841723), Provisional patent application filed 07-01-2013.